We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets.The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PPsecreting cells) were located in the periphery of the islets.However, A-cells and PP-cells had a different regional distribution.Most of Occassion Dresses A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas.In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas.
Furthermore, we found that Collections 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining.Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells.